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Image Search Results
Journal: Journal of neuroinflammation
Article Title: Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
doi: 10.1186/1742-2094-11-105
Figure Lengend Snippet: Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) CD206+Ym1+Arg1+ or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
Article Snippet: For macrophage subtypes and neutrophils, cells were stained brilliant violet (BV)711-labeled anti-mouse CD11b, PB-labeled Ly6G, PE-labeled Ly6C, PE-Cy7-labeled anti-mouse CD11c, PE-labeled IL-6, PE-Cy7 labeled TNFα, (BD Bioscience), APC-labeled F4/ 80, PE-labeled CD206, BV510-labeled CD86, PE-Cy7labeled CD45, APC-Cy7-labeled MHCII (Biolegend),
Techniques: Flow Cytometry, Quantitative RT-PCR, Isolation, Knock-Out, Control, Expressing